Human Epidermal Keratinocytes, 10-Donor Pool
Lifeline® normal Human Primary Epidermal Keratinocytes (HEK), when grown in Lifeline® DermaLife K Keratinocytes Medium, provide an ideal serum-free culture model, for the study of wound healing, toxicology or epithelial biology. When in need of multiple keratinocyte cell lots, our Epidermal Keratinocyte 10-donor pool offers a wide range of donor demographics for research at an…
Epidermal Keratinocytes — Neonatal, Primary
Lifeline® Normal Human Epidermal Keratinocytes Neonatal (HEKn), when grown in Lifeline DermaLife K Keratinocytes Medium, provides an ideal serum-free culture model, for the study of wound healing, toxicology or epithelial biology. Your experimental outcomes will be true: Primary Keratinocytes are directly cultured from their source organ tissue and have not been modified in any way. This…
Epidermal Keratinocytes — Adult, Primary
Lifeline® Normal Human Primary Epidermal Keratinocytes Adult (HEKa), when grown in Lifeline DermaLife K Keratinocyte Medium, provides an ideal serum-free culture model, for the study of wound healing, toxicology or epithelial biology. Primary Human Adult Keratinocytes are directly cultured from their source organ tissue and have not been modified in any way. This helps ensure…
Keratinocytes in Research: Epidermal Differentiation and Senescence
Function and Differentiation of Keratinocytes Keratinocytes make up the majority of cells in the epidermis, the outer layer of the skin. The progenitor cells that give rise to keratinocytes are found in the basal epidermal layer. As these cells proliferate and differentiate into keratinocytes, they move to the upper layers of the epidermis. Once they…
The Role of Hedgehog Receptors in Regulating the Epidermal Immune Response
National Healthy Skin Month emphasizes the importance of maintaining healthy skin as a key aspect of overall health and well-being. The skin barrier, specifically the epidermis, protects the body against environmental threats, such as pathogens and chemicals, while also helping to maintain internal homeostasis and preventing water loss from the body. Improving our understanding of…
Testing Fully-Automated Cell Tracking Algorithm Using Slow-Moving Keratinocytes
Keratinocytes to Study Cell Migration Keratinocytes are the most abundant cell type found in the epidermis, the outermost layer of the skin. Their primary function is to form a protective barrier against environmental damage as well as playing a key role in wound healing where they, along with endothelial and stem cells can migrate to…
Evaluating Chemotaxis of Epidermal Keratinocyte Migration
Keratinocytes: The Key Supporter of the Skin Barrier Keratinocytes are the most common cell type of the skin. They are present in the epidermis, which is the outermost layer of the skin, responsible for forming a barrier against the external environment and preventing water loss. The keratinocytes you see on your skin are mostly corneocytes,…
Melanocytes, Dermal Fibroblasts, and Keratinocytes – Protectors to the External World
The Skin: A Multi-Cellular Barrier to the External World The skin is a multi-layered organ that covers the entire body and protects us from the external environment. The outermost layer, the epidermis, is largely composed of keratinocytes, which proliferate in the lower epidermis and migrate out, becoming post-mitotic and losing their nuclei, eventually becoming corneocytes.…
Keratinocytes and Dermal Fibroblasts: External Layers for Internal Protection
The Multiple Layers and Cell Types of the Skin The skin is the ultimate barrier to the external environment, with a coverage area of the entire body. To perform its specialized functions, the skin is composed of multiple layers: the epidermis (outermost layer) and dermis (innermost layer). Below the dermis is the hypodermis, which is…
Keratinocytes and Melanocytes Skin Barrier Function Studies
The Barrier Function of the Skin Protects the Body from the External Environment The skin is the largest organ of the body and as the outer covering of the body, has a significant protective role. The outermost layer of the skin is called the epidermis, and is composed of multiple layers of keratinocytes. Keratinocytes proliferate…
Making Strides in Skin Cell Research Utilizing Lifeline® Keratinocytes and Melanocytes
Cells of the Skin: Keratinocytes and Melanocytes The skin has three layers, the epidermis (outermost), the dermis (middle), and the hypodermis (the innermost). The epidermis is largely composed of keratinocytes, which form a barrier against the external environment and play a role in wound repair. Melanocytes are another common cell type found in the skin.…
Keratinocytes: Barrier to the Outside World
The epidermis is the outermost layer of the skin, which covers the entire body and provides an essential barrier against the external environment. The most common epidermal cell type is the keratinocyte. Keratinocytes are born in the basal layer of the epidermis and migrate to the outer layers as they mature, becoming terminally differentiated. These…
Human Keratinocytes: First Line of Defense
Keratinocytes are the major cell type of the epidermis (the outermost skin layer) and are also found in the oral mucosa. They form the primary protective barrier between the internal milieu and the external environment. Keratinocytes are so named because they produce high amounts of the protein keratin, a fibrous protein that composes the protective…
Epidermal Cells – Our First Line of Defense — and Recent Studies
The skin is a multi-layered organ that covers the entire body and serves as a barrier to the outside environment. The outermost layer is called the epidermis and is composed largely of keratinocytes, which function to provide a barrier to pathogens, temperature, UV radiation, and other damaging environmental factors. Also contained in the epidermis are…
Keratinocytes: Guardians of the Skin!
Your skin is your strongest form of protection from a plethora of environmental insults. From bad weather, to pathogens, to UV exposure, to injury, skin is the barrier that not only keeps your tissues and organs in place, but also keeps your body safe from contamination and infection. Multiple cell types comprise the different layers…
Melanocytes and Keratinocytes Respond Differently to UV
Spring is on the way, and with it — more sunshine, and more reminders to wear sunscreen. There is a good reason to wear sunscreen: it protects against melanoma, the incidence of which has been increasing in recent years, making it a key public health concern. A group of researchers from the University of New Mexico…
Evaluation of a Broad-Molecular-Weight Hyaluronic Acid Complex Using Human Skin Models
Psoriasis Action Month Psoriasis is a chronic inflammatory skin disease that causes skin cells to multiply too quickly, resulting in dry, itchy, scaly plaques. In addition to inflammation, the skin barrier is often compromised, leading to increased transepidermal water loss (TEWL) and reduced moisture retention. While moisturizers and barrier-supporting ingredients do not treat psoriasis itself,…
Addressing Cell Culture Media FAQs
Primary human cells isolated directly from tissue contain biologically relevant information that is essential for addressing a wide range of research questions. These cells are widely used in studies of disease mechanisms, immunology, toxicology, and drug development because they more closely reflect in vivo physiology compared to immortalized cell lines. However, primary cells are more…
Addressing Primary Cell Culture FAQs
Cell culture is the process of growing cells under controlled conditions outside of their natural environment (e.g., organ systems of the body). By studying cells in vitro, researchers can examine cellular behavior in a highly controlled and reproducible way, providing insight into fundamental processes such as metabolism, signaling, and aging. Cell culture also enables systematic…