Lifeline® Normal Human Cardiac Fibroblasts provide an ideal cell system to establish serum-free human feeder layers for human embryonic stem cell cultures, or as a model to study wound healing, toxicology, or basic cell biology.
Lifeline® Cardiac Fibroblasts are cryopreserved as secondary cells — the cells have been isolated, plated and expanded in culture vessels twice before being harvested for cryopreservation — to ensure the highest purity, viability, and plating efficiency.
Our fibroblasts are quality tested in FibroLife® S2 or FibroLife® Xeno-Free Medium to ensure proper growth and morphology over a period of at least 15 population doublings.
Our Cardiac Fibroblasts are not exposed to antimicrobials or phenol red when cultured in Lifeline® FibroLife® medium. Lifeline offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation. A vial of Gentamicin and Amphotericin B (GA; LS-1104) is provided with the purchase of FibroLife® S2 (LL-0011) Medium Complete Kit for your convenience. The use of GA is recommended to inhibit potential fungal or bacterial contamination of eukaryotic cell cultures. Phenol Red (LS-1009) may be purchased, but is not required.