Accelerate Research Breakthroughs with Lifeline & BioSurfaces
Combine Lifeline® primary human cells and optimized media with BioSurfaces’ Bio‑Spun™ nanofiber scaffolds to unlock next‑generation 3D tissue models. Together, deliver unmatched physiological relevance, reproducibility, and agility to your in vitro research — so you can generate superior results, faster.
Why Use Lifeline & BioSurfaces Together?

Physiological Fidelity
Bio-Spun™ scaffolds mimic natural extracellular matrix (ECM) with high porosity and customizable nanofiber structures, promoting robust cell adhesion, function, and differentiation.

Premium Cell Quality
Lifeline supplies human-derived primary cells (keratinocytes, airway, bronchial, epithelial) with rigorously optimized serum-free media for reliable, reproducible culture outcomes.

Seamless Integration
Both product lines are designed to fit standard labware (e.g., inserts, plates), simplifying experimental setup. Bio-Spun™ scaffolds are available in formats that nest directly into common plates, and Lifeline cells are matched for scaffold seeding.

Faster Breakthroughs
High-quality cells + advanced scaffolds = physiologically relevant models that accelerate data quality, shorten timelines, and strengthen translational confidence.
Research Applications Where Lifeline + BioSurfaces Shine
APPLICATIONS
WHY THE COMBO WORKS
RESPIRATORY MODELS
Culture airway cells at the air–liquid interface on Bio-Spun™ scaffolds for in vivo-like exposures
SKIN & WOUND MODELS
Seed keratinocytes on flat nanofiber scaffolds to reconstruct full-thickness skin barriers
BARRIER TESTING
Investigate drug permeation or pathogen interaction across epithelial layers with consistent, reproducible models
CO-CULTURE SYSTEMS
Stack epithelial, stromal, or immune cells onto Bio-Spun™ matrices for complex model creation
Key Products for Integrated Research Workflows

BioSurfaces Products
Bio-Spun™ Scaffolds in 6-, 12-, and 24-well insert plates or 24- and 96-well HTS formats.
Custom-sized scaffolds for non-degradable and biodegradable polymers

Lifeline® Products
Human primary cells (keratinocytes, airway, bronchial, epithelial).
Serum-free media systems tailored for scaffold seeding and cell maintenance
Need Help Choosing the Right Setup? We’re Here for You.
Our expert teams at Lifeline® are ready to assist you in designing the ideal combination of scaffold, cell type, and media to meet your experimental needs. Whether you’re tackling barrier modeling, co-cultures, or high-throughput screening, we’ll guide you to the best workflow for reliable, translational results.

Joining Forces to Deliver Breakthrough Results

About BioSurfaces
BioSurfaces is a pioneering company specializing in Bio-Spun™ electrospun nanofiber technology. Their mission is to improve lives globally using nanofiber materials engineered into medical devices, research tools, and tissue scaffolds that outperform traditional solutions.

About Lifeline® Cell Technology
Lifeline® Cell Technology (Lifeline®) specializes in the development and manufacture of purified primary human cells and optimized reagents for cell culture. Lifeline® continues to be an industry leader by providing unequaled value through meticulous quality testing, constant innovation, and a passion to provide the best customer and technical care.