Lifeline® human cells include: endothelial, epithelial, fibroblasts, hematopoietic, melanocytes, smooth muscle, and mesenchymal stem cells. All of Lifeline® normal human and animal (mouse) cells:
Need not be exposed to antimicrobials or phenol red when cultured in Lifeline culture media. Antimicrobials and phenol red are not needed, and not recommended, to achieve optimal cell performance. These supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
Are negative for mycoplasma, and negative for bacterial and fungal growth.
Are negative for HIV, HBV, and HCB by PCR.
Primary cells are defined as cells that have been isolated from tissue, plated onto culture vessels, expanded, harvested and cryopreserved.
Secondary cells are defined as cells that have been isolated, plated, and expanded twice in culture vessels before being harvested for cryopreservation.
Optimized Media for Each Cell and Tissue Type
Lifelife® cells will grow faster in Lifeline® media than in any other commercially-available medium.