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Human fibroblasts include:  bladder, cardiac, dermal, lung-airway, gingival, uterine, and vas deferens.

Lifeline® normal Human Fibroblasts provide an ideal cell system to study wound healing, toxicology, cancer, or basic cell biology in various organs including skin, lung, bladder, and the reproductive systems. Our normal Human Fibroblasts can also be used for drug screening, drug development, and genome editing applications. Additionally, our fibroblast lines are ideal for establishing serum free human feeder layers for human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and other cell culture applications requiring feeder layers.

Human Dermal Fibroblasts (Adult or Neonatal) and Xeno-Free Human Dermal Fibroblasts are cryopreserved as primary cells.
Human Cardiac, Lung, Uterine, Vas Deferens, and Bladder Fibroblasts are cryopreserved as secondary cells to ensure the highest purity, viability and plating efficiency.

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Human Fibroblastsfibroblasts in the body-2

Our fibroblasts are quality tested in FibroLife® S2 or FibroLife® Xeno-Free Medium to ensure proper growth and morphology over a period of at least 15 population doublings.  Lifeline® fibroblasts demonstrate optimal growth at rates equal to or greater than classical serum-supplemented media.

Lifeline® fibroblasts need not be exposed to antimicrobials or phenol red when cultured in FibroLife® Medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

Cell Features

Human Fibroblast Cell Type Features

  • Lifeline® designates “primary cells” as cells that have been isolated from tissue, plated onto culture vessels, expanded, harvested and cryopreserved.
  • The term “secondary” indicates that the cells have been isolated, plated and expanded in culture vessels twice before being harvested for cryopreservation.

Quality Testing

Lifeline® Cell Technology manufactures products using the highest quality raw materials and incorporates extensive quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

Fibroblast Cell Features