Lifeline® Human CD14+ Monocytes provide an ideal culture model for the study of immunology. These human CD14+ Monocytes are purified from leukapharesed peripheral blood using immunomagnetic positive selection.
Normal CD14+ Monocytes are cryopreserved directly after isolation to ensure optimal phenotype and the highest viability and plating efficiency. Our Human CD14+ monocytes are quality tested via flow cytometry to ensure the enrichment of CD14+ cells. 5M cells per vial.
Lifeline CD14+ Monocytes are maintained in a suspension culture. Monocytes will not proliferate under maintenance conditions.
CD14+ Monocytes are not exposed to antimicrobials or phenol red. (Lifeline offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation.)
Use RPMI1640-FBS Medium Complete Kit with CD14+ Monocytes. The Kit includes frozen basal medium containing phenol red, 2 mM L-glutamine, 25 mM HEPES and 1 mM sodium Pyruvate. The Complete Kit also includes Fetal Bovine Serum and GA (Gentamicin and Amphotericin B).
Protocols & Instructions