Using Lifeline® Cells and Culture Media
Thank you for choosing Lifeline® Cell Technology. It is our goal to ensure your success when thawing and culturing our primary cells.
In order to avoid errors, Lifeline® recommends that you read the instructions included with the cell and medium products in their entirety.
Culture Medium Instructions
Warm medium in a water bath and mix well prior to use. When using small amounts of medium, Lifeline® recommends warming only the amount needed in a sterile conical tube.
To thaw the cells, hold the bottom of the vial in a 37-degree water bath for approximately one minute. A small ice chip should still be seen.
Spray the vial with 70% ethanol and transfer to biosafety cabinet. Gently re-suspend the cells using a sterile pipette. Do not centrifuge. The cells may be directly plated from the vial.
Perform a cell count using the hemocytometer and plate cells in a pre-warmed fully supplemented medium at the recommending seeding density. Then transfer the cells to a 37-degree incubator for storage.
If you have any questions, please contact technical service. We are here to help. Thank you.