RenaLife ™ Epithelial Medium Complete Kit

Product Description

RenaLife™ medium is optimized for the low-serum culture of Human Renal Epithelial Cells. RenaLife™ supports the growth of these cells over a period of at least 15 population doublings.

RenaLife™ Basal Medium contains no antimicrobials and no phenol red. These supplements are available from Lifeline^® but are not required for eukaryotic cell proliferation.

• Kit format for formulation flexibility:  RenaLife™ culture medium is offered in a kit format composed of RenaLife™ Basal Medium (500mL) and associated supplements and growth factors called “LifeFactors.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
• Protective packaging:  RenaLife™ Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.

ISO-Style Testing for Guaranteed Consistency and Reproducible Results

Every production lot of RenaLife™ Medium is extensively tested using Human Renal Epithelial Cells to ensure lot-to-lot consistency.

• Sterility Testing:  Negative for bacterial and fungal growth
• pH: 7.5 +/- 0.2
• Cell Testing: Rate of proliferation and morphology
• Osmolality: 315 +/- 10 mOsm
• Endotoxin: < 0.5 EU/mL

RenaLife^TM Medium grows Human Renal Epithelial Cells through at least 15 population doublings at rates that are comparable to other commercially available low-serum media, while maintaining more characteristic epithelial morphology, e.g., more compact cuboidal shape, high refractive index and clarity of the cytoplasm.

Shown left: Renal Cortical Epithelial Cells, third subculture (100X).

Shown below: gamma-Glutamyltransferase (GGT) assay. The substrate (gamma-glutamyl-p-nitroanilide) and acceptor molecule (glycylglycine) are incubated with the cells in a sodium phosphate buffer for 30 minutes at 37^°C. The released product is p-nitroaniline (PNA). The absorbance at 405 nm is measured and compared against a standard curve of PNA. Protein is quantified using a modified Bradford method.