Lifeline® Human CD4+ T Cells provide an ideal culture model for the study of immunology. These human CD4+ T Cells are purified from leukapharesed peripheral blood using immunomagnetic negative selection.
Human CD4+ T Cells are cryopreserved directly after isolation to ensure optimal phenotype and the highest viability and plating efficiency. Lifeline human CD4+ T Cells are quality tested via flow cytometry to ensure enrichment of cells that highly express the CD4 marker.
CD4+ T Cells are maintained in a suspension culture. They will not proliferate under maintenance conditions.
Lifeline® CD4+ T cells are not exposed to antimicrobials or phenol red. (Antimicrobials and phenol red are available for purchase, but they are not required for eukaryotic cell proliferation.)
- Human CD4+ Helper T Cells are cryopreserved at 5 x 106 cells per vial
- Human CD4+ Helper T Cells are extensively tested for quality and optimal performance
- Lifeline guarantees performance and quality
Use RPMI1640-FBS Medium Complete Kit with CD4+ T cells. The Kit includes frozen RPMI 1640 basal medium containing phenol red, 2 mM L-glutamine, 25 mM HEPES, 1 mM sodium Pyruvate. The Kit also includes Fetal Bovine Serum and GA (Gentamicin and Amphotericin B).
Protocols & Instructions