Lifeline® normal Human Corneal Epithelial Cells (HCEC), when grown in Lifeline® OcuLife™ Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HCEC are cell-matrix interactions, gene regulation and tissue development, drug development, and validation of alternative methods in toxicology.
- HCEC are isolated from human corneal tissue and expanded twice in culture vessels before being harvested for cryopreservation.
Lifeline® HCEC are not exposed to antimicrobials or phenol red when cultured in OcuLife™ Medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements, but they aren’t recommended or needed to achieve optimal cell performance in most research applications.
Human Corneal Epithelial Cells are tested for:
- Cell Count: 500,000 cryopreserved cells per vial
- Proliferation and Morphology in Fully Supplemented OcuLife™ Medium: Normal cell appearance for 3 passages
- Cell Viability: Minimum 70% viability when thawed from cryopreservation
- Sterility: Negative for mycoplasma, Negative for bacterial and fungal growth
- Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
500,000 cells per vial
Product Specification Sheets
Corneal Epithelial Cell Systems
SDS and “Safety Data Sheets”
(MSDS or Material Safety Data Sheets)