Mesenchymal stem cells (MSCs) are stromal cells that are primarily found in the bone marrow, but can be isolated from other locations, including adipose, the umbilical cord as well as fetal liver and lung.
MSCs can differentiate into multiple cell types, such as adipocytes, chondrocytes, osteoblasts, and myocytes.
Due to their multipotency, MSCs have been used as a therapeutic strategy to induce tissue repair following injury.
Current Research Using Lifeline® MSCs
Arsenic is known to have detrimental physiologic effects, particularly in cardiac and metabolic disease. Kiel et al. investigated the mechanism by which arsenic exerts its pathogenic effects in adipose tissue. Previous studies showed arsenic affects adipocyte differentiation; since adipose tissue renewal and differentiation is regulated by MSCs, this suggested MSCs could be an arsenic target population. The researchers used LifeLine® pre-adipocyte MSCs and induced adipocyte differentiation using AdipoLife DfKt-1 adipogenesis medium. They found that arsenic inhibited adipocyte differentiation and this effect was mediated through the Gi protein-coupled endothelin receptors. This study implicates MSCs as an arsenic target cell population that may be important for arsenic-induced pathogenesis.
Hao et al. explored the use of umbilical cord mesenchymal stem cells (UCMSCs) as a treatment for peri-implant bone defects following insertion of dental implants. Integration with the existing bone, called osteointegration, is crucial to prevent implant failure. The researchers induced peri-implant defects in beagle dogs and treated implants with LifeLine® UCMSCs derived from Wharton’s Jelly, a mucosal substance found in the umbilical cord. They found that UCMSCs increased osteointegration and healing of peri-implant bone defects, suggesting that UCMSCs may present a useful reparative agent in this context.
Lifeline® Mesenchymal Stem Cells and Media
Lifeline® MSCs can be maintained in vitro in an undifferentiated state, or induced to differentiate into adipocytes, chondrocytes, or osteoblasts using the corresponding differentiation medium:
- Human Mesenchymal Stem Cells — Adipose Derived
- Human Mesenchymal Stem Cells — Wharton’s Jelly
- Human Pre-Adipocyte Cells
- Human Mesenchymal Stem Cells — Bone Marrow Derived
- StemLifeTM MSC Complete Medium
- AdipoLife DfKt-1 Adipogenesis Medium
- ChondroLife Complete Chondrogenesis Medium
- OsteoLife Complete Osteogenesis Medium
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