Air-Liquid Interface Epithelial Differentiation Medium

Product Description

HBTEC Air-Liquid Interface Differentiation Medium is a new frozen medium composed and supplemented specifically for Primary Human Bronchial/Tracheal Epithelial Cells.

HBTEC is optimized for air-liquid interface mucociliary differentiation of Primary Human Bronchial/Tracheal Epithelial Cells.

HBTEC Air-Liquid Interface Differentiation Medium contains no antimicrobials and no phenol red. Antimicrobials are not required, but are recommended during differentiation due to the duration of culture and the frequency of handling of the cultures. Antimicrobials and phenol red may be purchased separately from Lifeline®.

HBTEC Air-Liquid Interface Differentiation Medium is offered as a convenient and easy to use, fully-supplemented complete medium, stored frozen to extend shelf life.

Shown top left: HBTEC Fixed on Day 21 of culture in HBTEC-ALI Complete Differentiation Medium supplemented with 0.1X PSA.  Nuclei are stained with Hoechst 33342 (blue), cilia are stained with mouse monoclonal anti-beta tubulin IV clone [ONS.1A6] and with secondary antibody goat anti-mouse lgG conjugated with Texas Red.

Shown top right: Trans Epithelial Electrical Resistance (TEER) Values of HBTEC differentiated with HBTEC-ALI Differentiation Medium or two competitor media. HBTEC cells grown in HBTEC-ALI Differentiation Medium consistently had higher TEER values than cells grown in competitor media, indicating a higher number of tight junctions and better integrity of the monolayer.