BronchiaLife™ Basal Medium is optimized specifically for the serum-free culture of Primary Human Airway Epithelial Cells (including Bronchial/Tracheal Epithelial, Lobar Epithelial, and Small Airway Epithelial). BronchiaLife is balanced and supplemented expressly for Primary Airway Epithelial Cells, to maximize that individual cell type’s life expectancy.
BronchiaLife medium supports the growth of these Primary Cells over a period of at least 15 population doublings.
BronchiaLife contains no antimicrobials and no phenol red. Lifeline offers these traditional supplements, but they are not required for eukaryotic cell proliferation. The associated supplements and growth factors for use with Primary Airway Epithelial Cells are available in BronchiaLife LifeFactors Kit.
- Protective packaging: BronchiaLife Basal Medium is provided in a light-protected bottle.
BronchiaLife Medium grows Primary Human Bronchial/Tracheal Epithelial Cells through at least 15 population doublings at rates that meet or exceed other commercially available media while maintaining excellent cell morphology. In comparisons with other commercially available media, BronchiaLife shows superior proliferation at different seeding densities.
Shown at left: Human Bronchial/Tracheal Epithelial Cells, passage 3, 4 days after inoculation with 5,000 cells/cm2 (100X).
Shown below: proliferation at low seeding density in 24-well plates. Higher O.D. 590 represents a greater number of cells per well and therefore better proliferation.
- Sterility: Negative for bacterial and fungal growth
- pH: 7.5 +/- 0.2
- Cell Testing: Rate of proliferation and morphology
- Osmolality: 315 +/- 10 mOsm
- Endotoxin: < 0.5 EU/mL
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