BronchiaLife™ Basal Medium is optimized for the serum-free culture of Human Airway Epithelial Cells (including Bronchial/Tracheal Epithelial, Lobar Epithelial, and Small Airway Epithelial).
BronchiaLife™ medium supports the growth of these cells over a period of at least 15 population doublings.
BronchiaLife™ contains no antimicrobials and no phenol red. Lifeline® offers these traditional supplements, but they are not required for eukaryotic cell proliferation.
- Protective packaging: BronchiaLife™ Basal Medium is provided in a light-protected bottle.
BronchiaLife™ Medium grows Human Bronchial/Tracheal Epithelial Cells through at least 15 population doublings at rates that meet or exceed other commercially available media while maintaining excellent cell morphology. In comparisons with other commercially available media, BronchiaLife™ shows superior proliferation at different seeding densities.
Shown at left: Human Bronchial/Tracheal Epithelial Cells, passage 3, 4 days after inoculation with 5,000 cells/cm2 (100X).
Shown below: proliferation at low seeding density in 24-well plates. Higher O.D. 590 represents a greater number of cells per well and therefore better proliferation.
- Sterility: Negative for bacterial and fungal growth
- pH: 7.5 +/- 0.2
- Cell Testing: Rate of proliferation and morphology
- Osmolality: 315 +/- 10 mOsm
- Endotoxin: < 0.5 EU/mL
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