AdipoLife™ DfKt™-1 Adipogenesis Medium is optimized for the differentiation of Human Adipose-derived Mesenchymal Stem Cells (HMSC-Ad) and Pre-Adipocytes (HNSC-PA) into adipocytes.
AdipoLife™ Basal medium contains no antimicrobials and no phenol red. Antimicrobials are not required, but are recommended during differentiation due to the duration of culture and the frequency of handling of the cultures. Antmicrobials and phenol red may be purchased separately from Lifeline®,
AdipoLife™ is offered in a kit format composed of basal medium and associated supplements and differentiation factors called DifFactors™. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance. AdipoLife™ Basal Medium is provided in a 100 mL bottle. The remaining DifFactors™ are packaged in a convenient gas-impermeable pouch for easy storage.
AdipoLife™ DfKt™-1 Adipogenesis Medium Complete Kit includes:
- AdipoLife™ Basal Medium 100 mL
- DifFactor™ 1 — 1.0 mL
- DifFactor™ 2 — 5.0 mL
In comparisons with commercially-available media, AdipoLife™ DfKt™-1 shows superior efficiency of differentiation as is demonstrated by the greater accumulation of lipid vesicles.
Shown above, left: Human Mesenchymal Stem Cells -Adult, passage 4, 4 days after inoculation with approximately 4,500 cells/cm2 (100X).
Shown above, middle: Human Mesenchymal Stem Cells -Adult differentiated into Adipocytes using AdipoLife™ DfKt™-1 and stained with Lifeline® Oil Red O Lipid Staining Kit, post 3 weeks differentiation (200X).
Shown above, right: Human Mesenchyma Stem Cells -Adult differentiated into Adipocytes using a competitor adipogenesis differentiation kit and stained with Lifeline® Oil Red O Lipid Staining Kit, post 3 weeks differentiation (200X).
ISO-Style Testing for Guaranteed Consistency and Reproducible Results
AdipoLife™ DfKt™-1 Adipogenesis Medium is quality tested for:
- Sterility: Negative for bacterial and fungal growth
- pH: 7.6 +/- 0.3
- Cell Performance: Rate of proliferation and morphology
- Osmolality: 255 +/- 15 mOsm
- Endotoxin: < 0.5 EU/mL