FibroLife S2 Fibroblast Medium Complete Kit


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SKU: LL-0011 Categories: , Tag: GTIN: 1249000000000

Product Description

Optimized for Culturing Human Fibroblasts and Mesenchymal Stem Cells-Pre-Adipocytes in a Low-Serum Environment:  FibroLife S2 supports the growth of these cells in a 2% serum environment at rates equal to or greater than comparable media supplemented with 2% to 10% FBS.

FibroLife Basal Medium contains no antimicrobials and no phenol red. Antimicrobials and phenol red are not required for eukaryotic cell proliferation, but are available from Lifeline® .

FibroLife S2 provides an ideal cell system to establish low-serum human feeder layers for stem cell culture or as a model to study wound healing, toxicology or basic cell biology.

Kit format for formulation flexibility:  Offered in a kit format comprised of FibroLife Basal Medium in a light-protected 500 mL bottle, and FibroLife S2 LifeFactors Kit with associated supplements and growth factors. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.

Human Fibroblast Low Serum Culture Medium

ISO-Style Quality Testing for Reproducible Results

FibroLife S2 medium is tested for:

  • Sterility: Negative for bacteria and fungal growth
  • pH: 7.4 +/- 0.2
  • Cell Performance:  Rate of proliferation and morphology
  • Osmolality:  270 +/- 10 mOsm
  • Endotoxin:  < 0.5 EU/mL

fibroblasts on fibrolife S2 sheetSuperior proliferation: FibroLife S2 medium grows fibroblasts and pre-adipocytes in low-serum conditions at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology. In comparisons with other commercially-available media, FibroLife S2 medium shows superior proliferation at different seeding densities.

Shown at left: Human Dermal Fibroblasts-Neonatal, passage 2, 5 days after inoculation with 2,000 cells/cm2 (100X)

Shown below: Human Dermal Fibroblasts-Neonatal inoculated at low seeding densities in 24-well plates. Higher fluorescence represents a greater number of cells per well, and therefore, better proliferation.

fibroblasts media comparison

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