VascuLife® EnGS-Mv-US Kit
LL-0086 VascuLife EnGS-Mv-US Complete Kit is the US EnGS alternative to LL-0004
Evaluating Isocyanate Toxicity using Skin Cells
Isocyanates are a family of highly reactive, low molecular weight chemicals widely used to produce polyurethane products such as adhesives, spray paints, insulation, resins, sealants, foams, and surface coatings. Currently,…
Human Aortic Cells in Research for National Heart Month
Evaluation of Biocompatible Materials for Cardiovascular Microsensors February is National Heart Month, a time to bring awareness to cardiovascular health and heart disease–the leading cause of death in men and…
Impact of E-Cigarette Smoke on Cardiovascular Function
Cigarette smoking is one of the primary risk factors for cardiovascular disease (CVD). In fact, the American Heart Association reports that smoking-related diseases are responsible for > 440,000 deaths in the US each year. Cigarette smoke contains hundreds of chemical toxicants, which can damage the cardiovascular system, and increase the risk of developing atherosclerosis. Atherosclerosis…
Endothelium Barrier Function Helps Mitigate Metastasis
Cancer Biology: Learnings from Transcriptomics Analysis Human Umbilical Vein Endothelial Cells, or HUVECs as they are more commonly known, are widely utilized as an in vitro model system for studying physiological and pathological processes of the vascular system. Endothelial cells line the inside of blood vessels and have a central role in angiogenesis, the formation…
Extracellular Vesicles and Breast Cancer Metastasis
Endothelial Cells, Microvascular Endothelial Cells, and Cardiac Cells Cardiac cells and endothelial cells are two of the most important cell types that make up the heart tissue and blood vessels of the body’s vascular system. Almost all tissues rely on a blood supply for nutrients and gas exchange, which is supplied by a complex network…
Quality Data Relies on Quality Reagents
The ability to culture cells in vitro has provided scientists with insight into many tissue and organ systems in the human body. In order to obtain high quality, reliable results, it is imperative that experiments are conducted using high-quality cell culture reagents. Of critical importance is the quality of cell culture media to ensure the…
Regenerative Medicine Research with Skeletal Muscle Satellite Cells
Stem Cells, Regenerative Medicine, and Muscle Tissue In many adult tissues, adult stem cells are responsible for maintaining the tissue and replacing any lost tissue. To do so, adult stem cells are generally multipotent, having the ability to differentiate into the multiple differentiated cell types of a given tissue. Perhaps the most important goal of…
Potential Target Treatment of Vascular Dysfunction in Diabetes
The Circulatory System: The Blood Delivery System The circulatory system delivers the blood and oxygen that all tissues of the body require for normal operation. In particular, the arteries, veins, and capillaries that transport the blood throughout the body are lined by a layer of endothelial cells, which help to maintain blood and vessel homeostasis.…
Lifeline Cell Culture Media Produces Optimal Results
The ultimate goal of culturing cells in vitro is to create a model system that mimics the in vivo environment as much as possible. Perhaps the most important part of developing a reliable in vitro system is the selection of culture media. Cell phenotypes and behaviors are directed by the composition of cell culture media.…
Gingival Fibroblast Compatibility and Cytotoxicity of Dental Cement Compositions
Fibroblasts: The Main Components of Connective Tissue Fibroblasts are specialized cells that are often identified by their spindle-like shape and expression of the protein Vimentin. They are found in connective tissue throughout the body and where they produce collagen and the extracellular matrix. Fibroblasts are also key players in inflammation and wound healing, and their…
Cardiac and Endothelial Cells: Promoting Vasculature Homeostasis
Endothelial and Cardiac Cells The cardiovascular system is operated by the heart and blood vessels, or vasculature. The heart is a muscular organ that provides the force to pump the blood throughout the body in a network of blood vessels (arteries, veins, and capillaries). These blood vessels are lined by a layer of endothelial cells,…
Human Cells Research Studies Using Lifeline Cells: 2017 Review
Lifeline® Cells in 2017 It’s the end of the year again and here on the Lifeline® Cell Technology blog you have been keeping up with the various ways researchers from many fields have used Lifeline® cells. We are going to do something a little different this week and highlight a few studies that have featured…
Keratinocytes and Melanocytes Skin Barrier Function Studies
The Barrier Function of the Skin Protects the Body from the External Environment The skin is the largest organ of the body and as the outer covering of the body, has a significant protective role. The outermost layer of the skin is called the epidermis, and is composed of multiple layers of keratinocytes. Keratinocytes proliferate…
Keratinocytes: Barrier to the Outside World
The epidermis is the outermost layer of the skin, which covers the entire body and provides an essential barrier against the external environment. The most common epidermal cell type is the keratinocyte. Keratinocytes are born in the basal layer of the epidermis and migrate to the outer layers as they mature, becoming terminally differentiated. These…
Research on Mesenchymal Stem Cells: Multipotent Progenitors
Mesenchymal stem cells (MSCs) are a component of the stroma in multiple tissues, including bone marrow, adipose tissue, the umbilical cord (or Wharton’s Jelly), and others. MSCs are multipotent adult stem cells, meaning they can differentiate into multiple cell types. MSCs lineages include: fat tissue, cartilage, and bone. Given their ability to differentiate into multiple…
Human Keratinocytes: First Line of Defense
Keratinocytes are the major cell type of the epidermis (the outermost skin layer) and are also found in the oral mucosa. They form the primary protective barrier between the internal milieu and the external environment. Keratinocytes are so named because they produce high amounts of the protein keratin, a fibrous protein that composes the protective…
Gingival Structure, Function, and the Latest Research
The gingiva (or gums) is the tissue that surrounds and protects the teeth and underlying bone. The gingiva is attached to the tooth, forming a seal that protects the underlying bone and helps provide a barrier against infection. Good oral hygiene keeps this seal intact, while bad oral hygiene can result in damage to the…
Ascorbic Acid LifeFactor — 0.5 mL
May be used with: FibroLife® basal medium and human fibroblasts VascuLife® basal medium and human smooth muscle cells VascuLife® basal medium and human endothelial cells DermaLife basal medium and human melanocytes (neonatal, adult)
rh Insulin LifeFactor 0.5 mL
Recombinant human insulin (rh Insulin) may be used with: UroLife™ A basal medium and human bladder apex epithelial cells FibroLife® basal medium and human fibroblasts VascuLife® basal medium and human smooth muscle cells OcuLife™ basal medium and human corneal epithelial cells DermaLife basal medium or DermaLife calcium-free basal medium and human keratinocytes DermaLife basal medium…