FibroLife® Fibroblast Serum Free Medium Complete Kit

$114.15

In stock

SKU: LL-0001 Category: Tag:

Product Description

FibroLife® Serum-Free is a defined medium optimized for the serum-free culture of fibroblasts.  FibroLife® Serum-Free supports the growth of these cells in a serum-free environment at rates equal to or greater than media supplemented with 2% to 10% FBS.

FibroLife® Basal Medium contains no antimicrobials and no phenol red.  Lifeline® offers antimicrobial and phenol red, but they are not required for eukaryotic cell proliferation.

FibroLife®  Serum-Free provides a perfect cell system to establish serum-free human feeder layers for stem cell culture or as a model to study wound healing, toxicology or basic cell biology.

Kit format:  FibroLife® Serum-Free is offered in a kit format composed of basal medium and associated supplements and growth factors called LifeFactors® .  This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.  FibroLife®  Basal Medium is provided in a light-protected 500 mL bottle. FibroLife® LifeFactors® Kit with growth factors and supplements is packaged in a convenient gas-impermeable pouch for easy storage.

Human Fibroblast Serum Free Medium

 

Serum Free Fibroblast Culture MediaFibroLife® Serum-Free Medium grows human fibroblasts at rates that meet or exceed other commercially available serum-containing and serum-free media while maintaining excellent cell morphology.

Shown at left: Human Dermal Fibroblasts-Neonatal, passage 2, 6 days after inoculation with 2,000 cells/cm2 (100X).

Shown below: Human Dermal Fibroblasts-Neonatal inoculated at low seedin densities in 24-well plates. Higher fluorescence represents a greater number of cells per well, and therefore, better proliferation.

Fibroblast Media Comparison

FibroLife® Serum-Free Medium has been quality tested for:

  • Sterility:  Negative for bacteria and fungal growth
  • pH:  7.4 +/- 0.2
  • Cell Performance:  Rate of proliferation and morphology
  • Osmolality:  270 +/- 10 mOsm
  • Endotoxin: < 0.5 EU/mL

Product Specification Sheets

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