VascuLife^® VEGF-Mv (containing Vascular Endothelial Growth Factor) is a medium optimized for the culture of Human Microvascular Endothelial Cells. VascuLife^® supports the rapid proliferation of these cells in a 5% serum environment.

VascuLife^® VEGF-Mv Medium grows Human Dermal Microvascular Endothelial Cells and other Human Microvascular Endothelial Cells at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology.

• In comparisons with other commercially available media, VascuLife^® shows superior proliferation at different seeding densities.

VascuLife^® Basal Medium contains no antimicrobials and no phenol red. Lifeline^® offers these traditional supplements, but they are not required for eukaryotic cell proliferation. VascuLife^® VEGF-Mv contains rh VEGF which may affect stimulation assays. Lifeline recommends using VascuLife^® EnGS-Mv for these studies.

VascuLife^® Cell Culture Medium Components

VascuLife^® is offered in a kit format composed of basal medium and associated supplements and growth factors called LifeFactors^®. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance. VascuLife^® Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors^® (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.  The LifeFactors® included are:

ISO-Quality Testing for Guaranteed Consistency and Reproducible Results

Every production lot of VascuLife® VEGF-Mv Medium is extensively tested using Microvascular Endothelial Cells to ensure lot-to-lot consistency.

• Sterility Testing:  Negative for bacteria and fungal growth
• pH:  7.8 +/- 0.3
• Cell Testing:     Rate of proliferation and morphology
• Osmolality:  270 +/- 10 mOsm
• Endotoxin:  < 0.5 EU/mL