VascuLife® VEGF-Mv (containing Vascular Endothelial Growth Factor) is a medium optimized for the culture of Human Microvascular Endothelial Cells. VascuLife® supports the rapid proliferation of these cells in a 5% serum environment.
VascuLife® VEGF-Mv Medium grows Human Dermal Microvascular Endothelial Cells and other Human Microvascular Endothelial Cells at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology.
- In comparisons with other commercially available media, VascuLife® shows superior proliferation at different seeding densities.
VascuLife® Basal Medium contains no antimicrobials and no phenol red. Lifeline® offers these traditional supplements, but they are not required for eukaryotic cell proliferation. VascuLife® VEGF-Mv contains rh VEGF which may affect stimulation assays. Lifeline recommends using VascuLife® EnGS-Mv for these studies.
VascuLife® Cell Culture Medium Components
VascuLife® is offered in a kit format composed of basal medium and associated supplements and growth factors called LifeFactors®. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance. VascuLife® Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors® (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage. The LifeFactors® included are:
ISO-Quality Testing for Guaranteed Consistency and Reproducible Results
Every production lot of VascuLife® VEGF-Mv Medium is extensively tested using Microvascular Endothelial Cells to ensure lot-to-lot consistency.
- Sterility Testing: Negative for bacteria and fungal growth
- pH: 7.8 +/- 0.3
- Cell Testing: Rate of proliferation and morphology
- Osmolality: 270 +/- 10 mOsm
- Endotoxin: < 0.5 EU/mL
