VascuLife® VEGF-Mv (containing Vascular Endothelial Growth Factor) is a medium optimized for the culture of Human Microvascular Endothelial Cells. VascuLife® supports the rapid proliferation of these cells in a 5% serum environment.
VascuLife® VEGF-Mv Medium grows Human Dermal Microvascular Endothelial Cells and other Human Microvascular Endothelial Cells at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology.
- In comparisons with other commercially available media, VascuLife® shows superior proliferation at different seeding densities.
VascuLife® Basal Medium contains no antimicrobials and no phenol red. Lifeline® offers these traditional supplements, but they are not required for eukaryotic cell proliferation. VascuLife® VEGF-Mv contains rh VEGF which may affect stimulation assays. Lifeline recommends using VascuLife® EnGS-Mv for these studies.
VascuLife® Cell Culture Medium Components
VascuLife® is offered in a kit format composed of basal medium and associated supplements and growth factors called LifeFactors. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance. VascuLife® Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage. The LifeFactors included are:
ISO-Quality Testing for Guaranteed Consistency and Reproducible Results
Every production lot of VascuLife® VEGF-Mv Medium is extensively tested using Microvascular Endothelial Cells to ensure lot-to-lot consistency.
- Sterility Testing: Negative for bacteria and fungal growth
- pH: 7.8 +/- 0.3
- Cell Testing: Rate of proliferation and morphology
- Osmolality: 270 +/- 10 mOsm
- Endotoxin: < 0.5 EU/mL
Product Specification Sheets
Endothelial Cell Systems
Skin Cell Systems