VascuLife® EnGS Endothelial Medium Complete Kit

Product Description

VascuLife^® EnGS (containing Endothelial Cell Growth Supplement) is a low-serum medium optimized for the culture of Human Endothelial Cells, including:

• Human Umbilical Vein Endothelial Cells (HUVEC)
• Human Aortic Endothelial Cells
• Other human large-vessel endothelial cells

VascuLife^® supports the growth of these cells in a low-serum environment without human VEGF.

VascuLife^® Basal Medium contains no antimicrobials and no phenol red.

• Kit format for formulation flexibility:  VascuLife^® EnGS culture medium is offered in a kit format composed of basal medium and associated supplements and growth factors called “LifeFactors^®.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
• Protective packaging: VascuLife^® Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors^® (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.

LifeFactors^® include:

ISO-Style Quality Testing for Guaranteed Consistency and Reproducible Results

• Sterility:  Negative for bacteria and fungal growth
• pH: 7.8 +/- 0.3
• Osmolality:  270 +/- 10 mOsm
• Endotoxin:  < 0.5 EU/mL

VascuLife^® EnGS Medium grows HUVEC and other human large-vessel endothelial cells, without the addition of human VEGF, at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology. In comparisons with other commercially available media, VascuLife^® shows superior proliferation at different seeding densities.

Shown at left: HUVEC, passage 2, 4 days after inoculation with 2,000 cells/cm² (100X).

Shown below: HUVEC inoculated at low seeding density in 24-well plates. Higher fluorescence represents a greater number of cells per well, and therefore, better proliferation.