VascuLife® EnGS Endothelial Medium Complete Kit


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SKU: LL-0002 Category: Tag:

Product Description

VascuLife® EnGS (containing Endothelial Cell Growth Supplement) is a low-serum medium optimized for the culture of Human Endothelial Cells, including:

  • Human Umbilical Vein Endothelial Cells (HUVEC)
  • Human Aortic Endothelial Cells
  • Other human large-vessel endothelial cells

VascuLife® supports the growth of these cells in a low-serum environment without human VEGF.

VascuLife® Basal Medium contains no antimicrobials and no phenol red.

  • Kit format for formulation flexibility:  VascuLife® EnGS culture medium is offered in a kit format composed of basal medium and associated supplements and growth factors called “LifeFactors®.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
  • Protective packaging: VascuLife® Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors® (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.

LifeFactors® include:

VascuLife endothelial culture medium EnGS

ISO-Style Quality Testing for Guaranteed Consistency and Reproducible Results

  • Sterility:  Negative for bacteria and fungal growth
  • pH: 7.8 +/- 0.3
  • Osmolality:  270 +/- 10 mOsm
  • Endotoxin:  < 0.5 EU/mL

endothelial cell culture

VascuLife® EnGS Medium grows HUVEC and other human large-vessel endothelial cells, without the addition of human VEGF, at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology. In comparisons with other commercially available media, VascuLife® shows superior proliferation at different seeding densities.

Shown at left: HUVEC, passage 2, 4 days after inoculation with 2,000 cells/cm2 (100X).

Shown below: HUVEC inoculated at low seeding density in 24-well plates. Higher fluorescence represents a greater number of cells per well, and therefore, better proliferation.

VascuLife Media Comparison

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