VascuLife® VEGF Endothelial Medium Complete Kit


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SKU: LL-0003 Category: Tag: GTIN: 1308000000000

Product Description

VascuLife® VEGF (containing Vascular Endothelial Growth Factor) is a low serum medium optimized for the rapid proliferation of Human Endothelial Cells, including:

  • Human Umbilical Vein Endothelial Cells (HUVEC)
  • Aortic Endothelial Cells
  • Other human large-vessel endothelial cells

VascuLife® culture medium supports the growth of these cells in a low serum environment.

VascuLife® Basal Medium contains no antimicrobials and no phenol red.

VascuLife® VEGF Medium contains rh VEGF, which may affect stimulation assays. Lifeline® recommends using VascuLife® EnGS for these types of studies.

  • Kit Format:  VascuLife® culture medium is offered in a kit format composed of basal medium and associated supplements and growth factors called ” LifeFactors .” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
  • Protective Packaging:  VascuLife® Basal Medium is provided in a light-protected 500 mL bottle.

LifeFactors include:

Human Endothelial Cell Culture Medium with VEGF

ISO-Style Quality Testing for Guaranteed Consistency and Reproducible Results

HUVEC cultured in VascuLifeVascuLife® VEGF medium grows HUVEC and other human large-vessel endothelial cells at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology. In comparisons with other commercially-available media, Vasculife® VEGF shows superior proliferation at different seeding densities.

Shown at left: HUVEC, passage 2, 4 days after inoculation with 2,000 cells/cm2 (100X).

Shown below: HUVEC inoculated at low seeding density in 24-well plates. Higher fluorescence represents a greater number of cells per well and therefore better proliferation.

Endothelial Cell Culture Media Comparison

Every production lot of VascuLife® VEGF Endothelial Cell Culture Medium is extensively tested using Endothelial Cells.

  • Sterility Testing:  Negative for bacteria and fungal growth
  • pH:  7.8 +/- 0.3
  • Cell Testing:  Rate of proliferation and morphology
  • Osmolality:  270 +/- 10 mOsm
  • Endotoxin:  < 0.5 EU/mL

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