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Normal Human Aortic Endothelial Cells, Secondary

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SKU: FC-0014 Categories: , , ID: 1170

Lifeline® normal Human Aortic Endothelial Cells, when grown in Lifeline® VascuLife® Medium, provide an ideal low-serum culture model — with or without human VEGF — for the accurate testing of compounds for the study of atherosclerosis or vascular biology.

  • Lifeline® Human Aortic Endothelial Cells are isolated in VascuLife EnGS Medium to ensure that they remain unexposed to recombinant human VEGF.
  • Aortic Endothelial Cells may be cultured with or without VEGF.
  • Our Aortic Endothelial Cells can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife® medium.

Lifeline® aortic cells are isolated from human aorta and cryopreserved in second passage to ensure the highest viability and plating efficiency.

  • Our human aortic epithelial cells are quality tested in VascuLife® EnGS 2% serum medium and demonstrate optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than other serum-supplemented media.

Lifeline®  aortic cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

500,000 cells per vial

Aortic Endothelial Cells have been quality tested for:

  • Proliferation and Morphology:  Normal cell appearance for 15 population doublings
  • Cell Viability:  Minimum 70% viability when thawed from cryopreservation
  • Sterility:  Negative for mycoplasma. Negative for bacterial and fungal growth
  • Virus:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR
  • Specific Staining:  von Willebrand Factor positive. Smooth muscle a-actin negative.