VascuLife® SMC Medium Complete Kit

$137.75

SKU: LL-0014 Category: Tag:

Product Description

VascuLife® SMC medium is optimized for the culture of Human Smooth Muscle Cells (SMC). VascuLife® SMC supports the growth of these cells over a period of at least 15 population doublings. VascuLife® Basal Medium contains no antimicrobials and no phenol red.

  • Kit format for formulation flexibility:  Offered in a kit format composed of VascuLife® Basal Medium (500 mL) and associated supplements and growth factors called the VascuLife® SMC LifeFactors® Kit.  This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
  • Protective packaging: VascuLife® Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors® (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.

VascuLife Smooth Muscle Cell MediumVascuLife® SMC Medium grows Human Smooth Muscle Cells through at least 15 population doublings at rates that meet or exceed other commercially available serum-containing media, while maintaining excellent cell morphology. In comparisons with other commercially-available media, VascuLife® shows superior proliferation at different seeding densities.

Shown at left: Human Aortic Smooth Muscle Cells, passage 2, 4 days after inoculation with 2,500 cells/cm2 (100X).

Shown below: Human Aortic Smooth Muscle Cells inoculated at low seeding density in 24-well plates. Higher fluorescence represents a greater number of cells per well and therefore better proliferation.

Smooth Muscle Cell Medium Comparison LifeFactors® (associated supplements and growth factors) included in the Kit are:

Human Smooth Muscle Cells Culture Medium

ISO-Style Quality Testing for Guaranteed Consistency and Reproducible Results

Lifeline® Cell Technology manufactures products using the highest quality raw materials and incorporates ISO-style quality assurance in every production run. Exacting standards and production procedures ensure lot-to-lot consistency. Every production lot of VascuLife® SMC Medium is extensively tested using Vascular Smooth Muscle Cells.

  • Sterility Testing:  Negative for bacteria and fungal growth
  • pH:  7.8 +/- 0.3
  • Cell Testing:  Rate of proliferation and morphology
  • Osmolality:  270 +/- 10 mOsm
  • Endotoxin:  < 0.5 EU/mL

 

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LL-0014