VascuLife® SMC Medium Complete Kit

Product Description

VascuLife^® SMC medium is optimized specifically for the culture of Primary Human Smooth Muscle Cells (SMC). VascuLife SMC supports the growth of these cells over a period of at least 15 population doublings. VascuLife Basal Medium contains no antimicrobials and no phenol red.

Primary Smooth Muscle Cells cultured in VascuLife SMC medium out-perform cells grown in any similar medium

You’ll get consistently reproducible results with VascuLife SMC medium, which is balanced and supplemented expressly for Primary Smooth Muscle Cells to maximize that individual cell type’s life expectancy.

• Your Primary Smooth Muscle Cells will plate better using VascuLife SMC optimized medium (as compared to our nearest competitors’ media or traditional high-serum media)
• Your Primary Smooth Muscle Cells will grow more quickly in VascuLife SMC medium than in other commercially-available media
• Primary Cells will maintain better morphology when cultured in VascuLife SMC medium

• Kit format for formulation flexibility:  Offered in a kit format composed of VascuLife Basal Medium (500 mL) and associated supplements and growth factors called the VascuLife SMC LifeFactors Kit.  This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
• Protective packaging: VascuLife Basal Medium is provided in a light-protected 500 mL bottle.

VascuLife SMC Medium grows Primary Human Smooth Muscle Cells through at least 15 population doublings at rates that meet or exceed other commercially available serum-containing media, while maintaining excellent cell morphology.

Superior proliferation: In comparisons with other commercially-available media, VascuLife shows superior proliferation at different seeding densities.Shown at left: Primary Human Aortic Smooth Muscle Cells, passage 2, 4 days after inoculation with 2,500 cells/cm2 (100X).

Shown below: Primary Human Aortic Smooth Muscle Cells inoculated at low seeding density in 24-well plates. Higher fluorescence represents a greater number of cells per well, and therefore better proliferation.

LifeFactors (associated supplements and growth factors) included in the Kit are:

ISO-Style Quality Testing for Guaranteed Consistency and Reproducible Results

Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO-style quality assurance in every production run. Exacting standards and production procedures ensure lot-to-lot consistency. Every production lot of VascuLife SMC Medium is extensively tested using Human Primary Vascular Smooth Muscle Cells.

• Sterility Testing:  Negative for bacteria and fungal growth
• pH:  7.8 +/- 0.3
• Cell Testing:  Rate of proliferation and morphology
• Osmolality:  270 +/- 10 mOsm
• Endotoxin:  < 0.5 EU/mL