Fibrotic Disease Research Using Human Dermal Fibroblasts
Fibroblasts and Wound Healing Fibroblasts are located in the connective tissue and produce the extracellular matrix, a supportive network that surrounds various cell types throughout the body. In addition to their role in tissue structure and support, fibroblasts also play a pivotal role in wound healing. The skin is a very dynamic organ that is…
Melanocytes, Dermal Fibroblasts, and Keratinocytes – Protectors to the External World
The Skin: A Multi-Cellular Barrier to the External World The skin is a multi-layered organ that covers the entire body and protects us from the external environment. The outermost layer, the epidermis, is largely composed of keratinocytes, which proliferate in the lower epidermis and migrate out, becoming post-mitotic and losing their nuclei, eventually becoming corneocytes.…
Keratinocytes and Dermal Fibroblasts: External Layers for Internal Protection
The Multiple Layers and Cell Types of the Skin The skin is the ultimate barrier to the external environment, with a coverage area of the entire body. To perform its specialized functions, the skin is composed of multiple layers: the epidermis (outermost layer) and dermis (innermost layer). Below the dermis is the hypodermis, which is…
Lifeline® Dermal Fibroblasts Utilized in Wound Healing Study
Fibroblasts: Important Players in Wound Healing Fibroblasts are a mesenchymal cell type, known for their spindle shape. Although they are present in most tissue types, they are often found in connective tissue due their function in producing extracellular matrix proteins. While they normally function to maintain homeostasis through extracellular matrix maintenance, they have a particularly…
Dermal Fibroblasts Used to Study Induced Pluripotent Stem Cells and UV Radiation
The skin is the body’s first level of defense against the external environment. It is composed of multiple tissue layers that are held together by connective tissue, which supports and maintains the tissue, and contains immune system components. Connective tissue in the skin is generated by dermal fibroblasts—specialized cells located in the dermis, the second…
Human Dermal Fibroblasts – Neonatal, Xeno-Free, Primary
Lifeline® Primary Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free (HDFn-XF), when grown in FibroLife® Xeno-Free Complete Medium, provide an ideal xeno-free culture system to establish xeno-free human feeder layers for human pluripotent stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. They have not been exposed to any non-human or…
Normal Human Dermal Fibroblasts — Adult, Primary
Lifeline® normal Human Primary Adult Dermal Fibroblasts (HDFa) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. Primary Dermal Fibroblasts are directly cultured from their source organ tissue and have not been modified…
Human Dermal Fibroblasts – Neonatal, Primary
Lifeline® Primary Normal Human Skin Fibroblasts – Neonatal (HDFn) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. Primary Dermal Neonatal Fibroblasts are isolated from neonatal human foreskin and cryopreserved after primary culture…
The Role of Hedgehog Receptors in Regulating the Epidermal Immune Response
National Healthy Skin Month emphasizes the importance of maintaining healthy skin as a key aspect of overall health and well-being. The skin barrier, specifically the epidermis, protects the body against environmental threats, such as pathogens and chemicals, while also helping to maintain internal homeostasis and preventing water loss from the body. Improving our understanding of…
Dental Implant Biomaterial and Myopia Research Using Lifeline’s Fibroblasts
Gingival and Scleral Fibroblasts Fibroblasts are a spindle-shaped cell present in the connective tissue of the body. Their main function is to produce elements of the extracellular matrix, a tissue support network composed of collagen, glycoproteins, and elastin. Human gingival fibroblasts are located in the periodontal tissue, or gums, in the mouth. They help to…
Evaluating Chemotaxis of Epidermal Keratinocyte Migration
Keratinocytes: The Key Supporter of the Skin Barrier Keratinocytes are the most common cell type of the skin. They are present in the epidermis, which is the outermost layer of the skin, responsible for forming a barrier against the external environment and preventing water loss. The keratinocytes you see on your skin are mostly corneocytes,…
Fibroblasts Studies: The Mitochondria and the Cytoskeleton, and Treating Yeast Biofilms
Fibroblasts are the main cell type in stromal tissue, the supporting tissue of an organ that contains structural and connective components. They are a multifunctional cell type whose main function is to secrete extracellular matrix, which forms the connective tissue of an organ. Fibroblasts play important roles in tissue homeostasis, and fibroblast dysregulation can play…
Fibroblasts: Roles in Homeostasis, Wound Healing, and Cancer
Fibroblasts are one of the most common types of stromal cells in the body and are responsible for generating extracellular matrix (ECM) components that support the tissue in which they exist. There is tremendous crosstalk between fibroblasts in the stroma and the epithelium or endothelium in a given organ, which is important in maintenance of…
Human Fibroblasts: a Multi-Faceted Cell Type
Fibroblasts are a type of mesenchymal cell most commonly found in the connective tissue of many organs, including the gastrointestinal tract, skin, bladder, uterus, and others. Fibroblasts produce connective tissue and extracellular matrix, both of which are important for supporting the organs in which they are produced. In addition, fibroblasts crosstalk extensively with surrounding tissues,…
Epidermal Cells – Our First Line of Defense — and Recent Studies
The skin is a multi-layered organ that covers the entire body and serves as a barrier to the outside environment. The outermost layer is called the epidermis and is composed largely of keratinocytes, which function to provide a barrier to pathogens, temperature, UV radiation, and other damaging environmental factors. Also contained in the epidermis are…
Fibroblasts: Oh, the Places You Will Go . . .
What are Fibroblasts? Fibroblasts are connective tissue cells that secrete the components that make up the extracellular matrix and the stroma. Both these structures provide a supportiv framework that keeps cells, tissues, and organs in their proper places. There are many different types of fibroblasts located in organs and tissues throughout the body. The main structural protein secreted by…
iPS Validated Fibroblasts
iPS Validated Fibroblast Cell Culture Systems Lifeline® Normal Human Dermal Fibroblasts have been validated for their ability to become Induced Pluripotent Stem Cells (iPS). Neonatal Fibroblast cells, LOT#00866, can be transduced with lentiviral vectors with very high efficiencies. Normal Human Dermal Fibroblasts derived from neonatal skin are cryopreserved as primary cells to ensure the highest…
Bidding 2025 Farewell
As 2025 comes to a close, Lifeline® Cell Technology is reflecting on a year growth, collaboration, and continued innovation. The global community of researchers using our primary cells and cell culture media systems for their scientific research continues to grow, and this year also marked several exciting milestones for our team. BioSurfaces Collaboration One highlight…