Product Description

Lifeline^® normal Human Dermal Fibroblasts – Adult (HDFa) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.

Lifeline^® Dermal Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency.

Our fibroblasts are quality tested in both FibroLife^® Serum-Free Medium and FibroLife^® S2 Medium to ensure:

• Optimal growth at rates equal to or greater than classical serum-supplemented medium
• Proper growth and morphology over a period of at least 15 population doublings.

500,000 cells per vial

Adult Dermal Fibroblasts are not exposed to antimicrobials or phenol red when cultured in the appropriate Lifeline® FibroLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

Dermal fibroblasts are quality tested for:

• Cell Viability:  Minimum 70% viability when thawed from cryopreservation
• Sterility Testing:  Negative for mycoplasma. Negative for bacterial and fungal growth.
• Virus Testing:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR