Human Dermal Fibroblasts – Neonatal, Primary


In stock

SKU: FC-0001 Categories: , GTIN: 1163000000000

Product Description

Lifeline® Normal Human Skin Fibroblasts – Neonatal (HDFn) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.

Lifeline® Dermal Fibroblasts – Neonatal are isolated from neonatal human foreskin and cryopreserved as primary cells to ensure the highest viability and plating efficiency.

  • Our skin fibroblasts are quality tested in FibroLife® S2 or FibroLife Xeno-Free Medium to ensure proper growth and morphology over a period of at least 15 population doublings.
  • Lifeline® dermal fibroblasts tested in both FibroLife® Serum-Free Medium and FibroLife® S2 Medium display optimal growth at rates equal to or greater than classical serum-supplemented medium.

500,000 cells per vial

Lifeline® dermal fibroblasts – neonatal are not exposed to antimicrobials or phenol red when cultured in the appropriate Lifeline FibroLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

Dermal fibroblasts are quality tested for:

  • Cell Viability:  Minimum 70% viability when thawed from cryopreservation
  • Sterility Testing:  Negative for mycoplasma. Negative for bacterial and fungal growth.
  • Virus Testing:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR


Product Specification Sheets

SDS and “Safety Data Sheets”

(MSDS or Material Safety Data Sheets)

Protocols & Instructions


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