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Normal Human Umbilical Vein Endothelial Cells (HUVEC), Primary

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$291.35

SKU: FC-0003 Categories: , , ID: 1165

Lifeline® normal Human Umbilical Vein Endothelial Cells (HUVEC), when grown in Lifeline® VascuLife® Medium, provide an ideal low serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology.

  • Lifeline® HUVEC may be cultured with or without VEGF.
  • HUVEC can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife® medium.

Lifeline® HUVEC are isolated from human umbilical cords, and cryopreserved as primary cells to ensure the highest viability and plating efficiency.

  • HUVEC are isolated in VascuLife® EnGS Medium to ensure that they remain unexposed to recombinant human VEGF.
  • Our HUVEC are quality tested in VascuLife® 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.
  • Lifeline® Umbilical Vein Endothelial Cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

500,000 cells per vial

Lifeline® umbilical vein endothelial cells have been quality tested for:

  • Proliferation and Morphology:  Normal cell appearance for 15 population doublings
  • Cell Viability:  Minimum 70% viability when thawed from cryopreservation
  • Sterility:  Negative for mycoplasma. Negative for bacterial and fungal growth
  • Virus:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR
  • Specific Staining:  von Willebrand Factor positive. Smooth muscle a-actin negative.

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FC-0003