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Human Coronary Artery Endothelial Cells, Tertiary

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SKU: FC-0032 Categories: , , ID: 1181

Lifeline® Normal Human Coronary Artery Endothelial Cells (HCAEC), when grown in Lifeline® VascuLife® Medium, provides an ideal culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology.

  • Coronary Artery Endothelial Cells are isolated in VascuLife® EnGS Medium to ensure that they remain unexposed to recombinant human VEGF.
  • Lifeline® Coronary Artery Endothelial Cells may be cultured with or without VEGF.
  • Coronary Artery Endothelial Cells can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife® medium.

Lifeline® HCAEC are isolated from human coronary arteries and cryopreserved as tertiary cells to ensure the highest viability and plating efficiency.

  • Our HCAEC are quality tested in VascuLife® EnGS medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than other serum-supplemented medium.
  • Human Coronary Artery Endothelial Cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

500,000 cells per vial

Lifeline® Coronary Artery Endothelial Cells have been quality tested for:

  • Proliferation and Morphology:  Normal cell appearance for 15 population doublings
  • Cell Viability:  Minimum 70% viability when thawed from cryopreservation
  • Sterility:  Negative for mycoplasma. Negative for bacterial and fungal growth
  • Virus:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR
  • Specific Staining:  von Willebrand Factor positive. Smooth muscle a-actin negative.