Lifeline® normal Human Small Airway Epithelial Cells (HSAEC), when grown in Lifeline® BronchiaLife™ Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing.
Airway epithelial cells are cryopreserved after primary culture, e.g., cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation.
Lifeline® small airway epithelial cells are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HSAEC are quality tested in BronchiaLife™ Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media.
HSAEC are not exposed to antimicrobials or phenol red when cultured in BronchiaLife™ medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
HSAEC are tested for:
- Proliferation and Morphology: Normal cell appearance for 15 population doublings
- Cell Viability: Minimum 70% viability when thawed from cryopreservation
- Sterility: Negative for mycoplasma Negative for bacterial and fungal growth
- Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
500,000 cells per vial