FibroLife® Xeno-Free Complete Frozen Medium is a variation of our FibroLife® cell culture medium optimized for culture of fibroblasts without the use of animal-derived components. FibroLife® Xeno-Free supports the growth of these cells in a 2% human serum environment at rates equal to or greater than comparable media supplemented with 2% to 10% FBS.
FibroLife® Xeno-Free Complete Frozen Medium contains all the necessary growth factors to support fibroblast growth. FibroLife® Xeno-Free contains no antimicrobials and no phenol red. (A vial of Xeno-Free Penicillin/Streptomycin is included in FibroLife® Xeno-Free Medium Complete Kit LL-0048.) Xeno-Free phenol red is not available at this time.
FibroLife® Xeno-Free provides an ideal cell system to establish Xeno-Free human feeder layers for stem cell culture or as a model to study wound healing, toxicology or basic cell biology.
FibroLife® Xeno-Free Complete Frozen Medium is offered as a fully supplemented frozen medium with extended shelf life of up to 2 years.
Xeno-Free trypsinization and freezing reagents are also offered by Lifeline®, to allow you to maintain a Xeno-Free culture environment.
FibroLife® Xeno-Free Complete Frozen Medium grows fibroblasts at rates that exceed other commercially-available media containing animal-derived components while maintaining excellent cell morphology. In comparison with FibroLife® S2, which outperforms other commercially-available fibroblast media, FibroLife® Xeno-Free Medium shows superior proliferation at different seeding densities.
FibroLife® Xeno-Free Complete Frozen Medium is quality tested for:
- Sterility: Negative for bacteria and fungal growth
- pH: 7.5 +/- 0.3
- Cell Performance: Rate of proliferation and morphology
- Osmolality: 255 +/- 15 mOsm
- Endotoxin: < 0.5 EU/mL